Karyotyping is a well-established method of investigating the genetic content of product of conceptions (POCs). Because of the high rate of culture failure and maternal cell contamination, failed results or 46,XX findings are often obtained. Different molecular approaches that are not culture dependent have been proposed to circumvent these limits. On the basis of the robust experience previously obtained with bacterial artificial chromosomes (BACs)-on-Beads™ (BoBs™), we evaluated the same technology that we had used for the analysis of prenatal samples on POCs.
KaryoLite™ BoBs™ as a ‘first-tier’ test in combination with other approaches showed beneficial, cost-effective and clearly enhanced POC testing.
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Grati FR1, Gomes DM, Ganesamoorthy D, Marcato L, De Toffol S, Blondeel E, Malvestiti F, Loeuillet L, Ruggeri AM, Wainer R, Maggi F, Aboura A, Dupont C, Tabet AC, Guimiot F, Slater HR, Simoni G, Vialard F.
1Research and Development, Cytogenetics and Molecular Biology, TOMA Advanced Biomedical Assays, Busto Arsizio, Varese, Italy. fgrati@tomalab.com
Prenat Diagn. 2013 Jan;33(1):32-41.
KaryoLite BoBs: un nuovo metodo con elevata resa diagnostica per indagare i tessuti abortivi.